副猪嗜血杆菌qseC基因缺失突变对生物被膜形成的影响

doi:10.11843/j.issn.0366-6964.2017.08.014

畜牧兽医学报 ›› 2017, Vol. 48 ›› Issue (8): 1499-1504.doi: 10.11843/j.issn.0366-6964.2017.08.014

副猪嗜血杆菌qseC基因缺失突变对生物被膜形成的影响

杨开杰, 冯賽祥, 周琪, 张建民, 廖明*, 樊惠英*

华南农业大学兽医学院, 广州 510642

收稿日期:2017-02-13 出版日期:2017-08-23 发布日期:2017-08-23
通讯作者: 樊惠英,女,教授,E-mail:hyfan@scau.edu.cn;廖明,教授,E-mail:mliao@scau.edu.cn
作者简介:杨开杰(1992-),男,湖北云梦人,硕士生,主要从事动物传染病学的研究,E-mail:scauykj@163.com
基金资助:
农业部公益性行业科研专项经费项目（201303034）

Effect of qseC Gene Mutation of Haemophilus parasuis on Biofilm Formation

YANG Kai-jie, FENG Sai-xiang, ZHOU Qi, ZHANG Jian-min, LIAO Ming*, FAN Hui-ying*

College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China

Received:2017-02-13 Online:2017-08-23 Published:2017-08-23

摘要/Abstract

摘要：

通过构建副猪嗜血杆菌qseC突变体，探讨qseC基因的缺失对副猪嗜血杆菌生长和生物被膜形成的影响。利用自然转化方法构建副猪嗜血杆菌qseC基因的突变体，测定副猪嗜血杆菌野生株SC096与缺失株△qseC的生长情况并绘制生长曲线。结晶紫染色法定性检测野生株和突变体生物被膜的差异；通过96微孔板定量方法检测突变体生物被膜的量；电镜观察二者生物被膜的变化。结果如下：成功构建qseC基因突变体。生长曲线显示副猪嗜血杆菌野生株SC096与缺失株△qseC生长速度基本一致。缺失株△qseC较之野生株SC096生物被膜的形成能力有所减弱。研究表明，qseC基因对副猪嗜血杆菌生物被膜的形成有重要的调控作用。

Abstract:

The qseC gene mutation of Haemophilus parasuis was constructed to investigate the effect of qseC gene on the growth and biofilm formation of H. parasuis. The qseC mutant of H. parasuis was constructed by natural transformation. The growth curves of wild-type strain SC096 and △qseC were depicted. Then crystal violet staining was used to test biofilm formation of wild-type strain and qseC mutant. The amount of biofilm of SC096 and △qseC were measured by a 96-well plate quantitative method. The biofilm changes of the two strains were then observed by scan electron microscope. Results were as follows:The qseC gene mutant of H. parasuis SC096 was constructed successfully. The growth curves revealed that the growth rate of SC096 was similar to that of its qseC mutant. The biofilm formation ability of △qseC was weaker than that of the wild type strain SC096. The results showed that qseC gene may play an important role in the regulation of H. parasuis biofilm formation.

中图分类号:

S852.613

杨开杰, 冯賽祥, 周琪, 张建民, 廖明, 樊惠英. 副猪嗜血杆菌qseC基因缺失突变对生物被膜形成的影响[J]. 畜牧兽医学报, 2017, 48(8): 1499-1504.

YANG Kai-jie, FENG Sai-xiang, ZHOU Qi, ZHANG Jian-min, LIAO Ming, FAN Hui-ying. Effect of qseC Gene Mutation of Haemophilus parasuis on Biofilm Formation[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2017, 48(8): 1499-1504.

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副猪嗜血杆菌qseC基因缺失突变对生物被膜形成的影响

Effect of qseC Gene Mutation of Haemophilus parasuis on Biofilm Formation

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